Inx2 protein is linearly distributed in some salivary gland cells.
A, Inx2 distribution in salivary gland cells located near the salivary duct. The optical section focuses on the basal (non-lumenal) end of the cells where putative annular junction vesicles are normally observed (Annular junctions). The salivary gland is from a wild type pupa. Inx2 protein was detected using a rabbit polyclonal anti-Inx2 primary and anti-rabbit FITC-conjugated secondary antibody. Asterisks mark Inx2 at the intercellular boundary and arrows point to each end of linear Inx2 patterns within the cell. B, The GFP-tagged endogenous α subunit of the Na+/K+ ATPase (allele ATPαG00109 described by Morin et al, 2001) also exhibits a linear distribution (arrows) in these salivary gland cells. Again, the optical section focuses on the basal end of the cells. The linear arrangement of the sodium pump component and Inx2 suggests that both possibly interact with the actin cytoskeleton (...they may even interact with each other and associate with the cytoskeleton via other unidentified proteins).