Ogre protein expression in larval eye and leg discs.
Ogre protein was detected using a specific anti-Ogre rabbit polyclonal primary antibody and an FITC-conjugated anti-rabbit fluoresent secondary antibody. Eye and leg discs are from wandering third instar wild type larvae. Ogre protein appears to be present in most, if not all, cells in the discs. Transcripts of all members of the Drosophila innexin gene family have previously been reported to be expressed throughout the wing disc Stebbings et al, 2002 and antibody staining confirms the presence of Ogre, Inx2 and Inx3 (Innexins in the wing disc). Escaper males carrying ogrelethal alleles exhibit no obvious external morphological phenotypes on wings, legs or eyes so Ogre appears to be redundant (...or at least can be compensated for...) in these developing tissues.