Ogre protein distribution in the wild type adult optic lobe.
A single optical section taken through the optic lobe of an adult fly expressing UAS-mCD8:GFP (green) under the transcriptional control of the nrv2GAL4 driver line (Sun et al, 1999). The nervous system has been antibody stained using a rabbit polyclonal anti-Ogre-specific primary antibody (red). The gain used when scanning the Ogre signal was set very high in order to show nearly all regions of the optic lobe where Ogre protein is present. Ogre distribution overlaps extensively with the expression pattern of nrv2GAL4. Loss of Ogre leads to adult optic lobe defects (example here) which can be partially rescued by expressing a rescue contruct, UAS-ogre, in the nrv2GAL4 pattern (Rescue of ogre optic lobe phenotype). Ogre is required in other parts of the central nervous system also (see Innexins in the nervous system). A, nrv2GAL4 expression pattern. B, Ogre protein distribution. C, Merged image.