Glia ensheathing the mushroom body pedunculus in a wild type brain.
The processes of neuropile glia ensheath the mushroom body (MB) pedunculus - the axonal tract connecting Kenyon cell input regions (calyx at the dorsal posterior side of the brain) to the ouput structures (lobes at the brain anterior). The animation shows a three-dimensional reconstruction of a small region of the pedunculus of a wild type animal (diagram showing the scanned region). The reconstruction was created from 100 optical serial sections using 'Volocity' software. Glial processes express UAS-mCD8::GFP.L (Green) under the transcriptional direction of the nrv2-GAL4 line (Sun et al,1999). Axons of the pedunculus tract have been stained with antibodies against Fasiclin II (purple). The innexins Ogre (Ogre in adult brain glia), Inx2 (Inx2 in MB ensheathing glia) and Inx3 can be detected in the ensheathing glia and Ogre is essential either for its development or maintenance. The glial sheath is severely reduced in an ogre mutant (images and animation) and defects are also observed in mushroom body axonal morphology even though these neurons do not express Ogre.