Inx2 protein distribution in the adult central nervous system.
Images of an essentially wild type adult brain showing three distinct optical sections; A,B,C - showing a region near the anterior of the central complex; D,E,F - region near the centre of the central complex; G,H,I - focusing near the posterior of the brain. The grey box in the brain diagram at the top of the image illustrates the region of the brain that was scanned at all three optical depths. Inx2 (purple) was detected using a rabbit polyclonal anti-Inx2 primary antibody and anti-rabbit Cy5 conjugated secondary antibody (A,D,G). UAS-mCD8:GFP (green) expression was driven in glial cells by the nrv2GAL4 line (Sun et al, 1999) (B,E,H). Merged images showing Inx2 and glia are presented in C,F,I and reveal that the most intense Inx2 staining is found around the periphery of the brain and also in regions that are occupied by neuropile glial processes within the brain. Ogre is also present in neuropile glia (Ogre in the adult brain) and these two innexin subunits appear to co-localise and could possibly interact in these cells (Inx2 and Ogre in neuropile glia). EB - ellipsoid body of the central complex. Ped - mushroom body pedunculus, seen as a cross section. α - tip of mushroom body α-lobe. No - noduli of the central complex. Cx - mushroom body calyx. PN - strong linear Inx2 staining at one edge (arrow) of a putative perineurial cell.