Inx2 protein distribution in neuropile glia ensheathing the mushroom bodies.
The image shows a 3D reconstruction generated from serial confocal images through the glial cylinder ensheathing the anterior end of the pedunculus (see Inx2 in brain glia for position). Inx2 protein occupies distinct 'clusters' within the mass of neuropile glial processes that ensheath the pedunculus - the long axonal tract linking Kenyon cell bodies on the dorsal posterior of the brain to the lobes at the anterior of the brain (see mushroom body morphology). Ogre is also present in neuropile glia (Ogre in the adult brain) and these two innexin subunits appear to co-localise and could possibly interact in these cells (Inx2 and Ogre in neuropile glia). In the image above Inx2 (red) is detected using a rabbit polyclonal anti-Inx2 primary antibody and anti-rabbit Cy5 conjugated secondary antibody. UAS-mCD8:GFP (green)expression is driven in glial cells by the nrv2GAL4 line (Sun et al, 1999).