Inx3 protein distribution in the posterior gut of an inx2null mutant embryo.
In the image above Inx3 (green) is detected using a rabbit polyclonal anti-Inx3 primary antibody and anti-rabbit FITC conjugated secondary antibody. The embryo is a hemizygote of genotype inx2F43 / Y which has a 1368bp deletion starting 32bp upstream of the predicted transcription start site and includes most of the inx2 protein coding sequence. No Inx2 protein is detected in such embryos and Inx3 becomes distributed over the plasma membrane (white arrow) and possibly within the cytoplasm in contrast to the intense punctate staining detected in wild type embryos. Some strongly staining Inx3-immunopositive punctae can be detected (red arrow) possibly reflecting perduring plaques containing Inx3 that oligomerized with Inx2 generated from maternally inherited mRNA.