Inx2 protein level changes with developmental stage within the salivary gland tissue.
Comparison of Inx2 protein levels between salivary glands from 3rd instar larvae (L3) and pupae. A, example of 6 glands from L3 larvae and 6 from pupae that were identically stained, mounted and scanned. L3 and pupal glands were identified post-scanning based on presence/absence of glue granules (present in L3 and absent in pupal tissues). All images are focused on the basal (non-lumen) end of salivary gland cells. B, a closer look reveals barely detectable punctate Inx2 staining in L3 stage glands but large intensely staining vesicles (putative annular junctions) near the surface of pupal glands. Surface plots of cell boundary regions (CB) confirm that the average vesicle intensity in L3s (32.0+13.8) is approximately half of the pupal intensity (66.6+11.1). Pupal vesicles are also consistently larger than larval vesicles. All size bars are 20μm.