Innexin Gap Junctions

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Secretory vesicles are abundant near the cell boundary only where Innexin staining is weak or absent.


Secretory vesicles are abundant near the cell boundary only where Innexin staining is weak or absent.

Optical sections at different cell-depth across the boundary of two pupal salivary gland cells. At the basal (non-lumenal, Z-section 1) end of cells large Inx2 (red) vesicles (putative annular junctions) are observed within the cell. Synaptobrevin-GFP (syb-GFP) marked secretory vesicles (green) are also present, and often cluster near Inx2 vesicles, but Inx2 and syb-GFP generally do not overlap. Relatively few syb-GFP secretory vesicles can be seen at the intercellular boundary at the basal end of cells (Z-section 1) or where the plasma membrane is occupied by Inx2 plaques (Z-section 2). Where Inx2 plaques are absent, toward the apical end of cells, syb-GFP vesicles accumulate at, or near, the intercellular boundary membranes (arrows, Z-section 3). The secretory vesicle marker used was P{w[+mC]=UAS-n-syb.eGFP}2 expressed under the transcriptional regulation of C96-GAL4. Size bar = 5μm.



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