Inx2 protein in a single pupal salivary gland cell.
A, Projection of a series of confocal Z sections through a single pupal salivary gland cell from the apical to the basal surface. Actin, phalloidin rhodamine (red); Inx2, FITC (green); Nuclei, DAPI (blue). B, 100 sections are projected onto a single plane to produce image A above. Innexin staining appears to be continuous around the cell plasma membrane in projected images. However, this is misleading. Single Z sections (shown elsewhere) reveal that innexin protein distribution is discontinuous in the plasma membrane indicating the presence of discrete gap junction plaque structures.