Inx2 protein, Na+/K+ ATPase α subunit and Fasiclin III location in embryonic salivary gland cells.
Inx2 exhibits a restricted apico-basal distribution in late embryo salivary gland cells. A, Whole embryonic salivary gland, antibody stained to detect Inx2 (blue) and Fasciclin III (red). The location of endogenous Na+ / K+ ATPase α subunit is revealed by ATPα-GFP expression (green). All of these proteins are found at high levels near the apical end of cells. A dashed white line indicates the outer edge of the salivary gland. B, Close-up view of some salivary gland cells, focusing on the apical end, next to the lumen (marked by *). Putative Inx2 plaques are located slightly basal to the other apical markers (ATPα-GFP and FasIII). Size bar in B is 5μm. The ATPα-GFP reporter line is AtpαG00109.