Schematic representation of Innexin protein distribution in pupal salivary gland cells.
Innexin protein is found at three distinct cellular locations. 1/ small vesicles near the nucleus (black). Possibly sites of innexin protein synthesis or transport vesicles. 2/ plaques at the plasma membrane (red). 3/ larger vesicles at the cell surface (green). Presumably annular junctions (internalized gap junctions). The diagram is based on observations made on antibody-stained wild type pupal salivary gland preparations using primary antibody against Ogre, Inx2 and Inx3 protein. The location of plaques and other gap junction elements described here differs from innexin distributions observed in other tissues (such as epidermis and amnioserosa cells) and even in the same tissue at different developmental stages (for example, embryonic versus pupal salivary gland cells).