Whole embryo Inx3 staining – wild type embryo.
Fluorescent images of an 8-10 hour old wild type embryo. A, Focusing on the embryo outer surface. Inx3 protein is abundant in cells of the epidermis (Ep) and can be seen along the edges of amnioserosa cells (As). B, An optical section from the middle of the embryo. Inx3 is strongly expressed in the epidermis (Ep) and the gut. Even at this level of magnification the punctate distribution of Inx3 can be resolved, particularly in the gut. Inx3 protein is also found in salivary glands, but they are not easily distinguished in this image. The embryo was antibody-stained to detect Inx3 protein using anti-Inx3 rabbit polyclonal primary antibody and an FITC-conjugated anti-rabbit secondary (green).