Inx3 protein distribution in amnioserosa cells.
The image shows amnioserosa cells (As) on the dorsal surface of an 8-10 hour old wild type embryo prior to dorsal closure. Inx3 accumulates at discrete plaques at the plasma membrane (arrows) and strongly resembles plaques produced by Ogre and Inx2. It is possible that there is some interaction between these three innexins in amnioserosa cells. The detected pattern of Inx3 protein in these cells differs markedly from that observed in dominant Inx2TA181 and inx2null mutant embryos. Inx3 protein (green) is detected using anti-Inx3 rabbit polyclonal primary antibody and an FITC-conjugated anti-rabbit secondary.