Innexin Gap Junctions

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Whole embryo Inx2 staining – wild type embryo.

Whole embryo Inx2 staining – wild type embryo.

Fluorescent images of an 8-10 hour old wild type embryo. The embryo was antibody-stained to detect Inx2 protein using anti-Inx2 rabbit polyclonal primary antibody and an FITC-conjugated anti-rabbit secondary (green). A, Focusing on the embryo outer surface. Inx2 protein is abundant in cells of the epidermis (Ep) and can be seen along the edges of amnioserosa cells (As). B, An optical section at the middle of the embryo. Inx2 is strongly expressed in the epidermis (Ep), the gut, and salivary glands (Sal). Even at this level of magnification the punctuate distribution of Inx2 can be resolved, particularly in the gut.

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