Innexin Gap Junctions

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Whole embryo Inx3 staining – dominant Inx2 mutant.

Whole embryo Inx3 staining – dominant Inx2 mutant.

The images show a fluorescent image of an 8-10 hour old embryo. Genotype Inx2TA181/Y. The embryo was antibody-stained to detect Inx3 protein using anti-Inx3 rabbit polyclonal primary antibody and an FITC-conjugated anti-rabbit secondary (green). A, Focusing on outer epidermal cells one can see that Inx3 protein is abundant in these cells, just as it is in wild type animals. No obvious defects in the epidermis are apparent even though no wild type Inx2 protein is produced. This contradicts some published reports describing holes in the epidermis of inx2 null embryos ( Bauer et al. 2004, Gap junction channel protein Innexin 2 is essential for epithelial morphogenesis in the Drosophila embryo). B, An optical section taken near the middle of the embryo. Inx3 is strongly expressed in the epidermis (Ep), the gut, and salivary glands (Sal).

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