Inx3 protein distribution in dominant Inx2TA181 mutant gut cells.
The image shows cells of the posterior embryonic gut of an 8-10 hour old embryo of genotype Inx2TA181 / Y. Inx3 protein (green) is detected using anti-Inx3 rabbit polyclonal primary antibody and an FITC-conjugated anti-rabbit secondary. The Inx3-staining intensity observed in cells of this genotype always appears to be low, presumably because the protein is distributed around the cell membrane and does not aggregate into the brightly fluorescent plaques observed in wild type animals. This distribution of Inx3 protein is almost identical to that observed in mutant embryos that produce no Inx2 protein at all. However, the Inx2TA181 allele appears to produce a roughly similar amount of Inx2 protein as wild type animals. This image confirms published observations ( Lehmann et al. 2006, Heteromerization of Innexin Gap Junction Proteins Regulates Epithelial Tissue Organization in Drosophila) that normal Inx3 subcellular localisation is dependent on wild type Inx2 protein.