Inx2 protein distribution in a dominant Inx2 mutant embryo.
Images from the same Inx2 mutant embryo. A, focusing on the epidermis (Ep). Mutant Inx2 protein is expressed strongly in cells of the epidermis. B, focusing on the interior of the embryo. Mutant Inx2 protein is abundant in the Gut and Epidermis (Ep) – all of the same places it is found in wild type embryos. The embryo is 8-10 hours after egg laying at 25C. Genotype is Inx2TA181/Y. Antibody staining using anti-Inx2 rabbit polyclonal primary antibody and an FITC-conjugated anti-rabbit secondary (green). As – Amnioserosa. The dominant Inx2 mutants were initially referred to as Stout alleles when they were first reported ( Florence and McGinnis, 1998, A Genetic Screen of the Drosophila X Chromosome for Mutations That Modify Deformed Function).