Inx2 distribution in amnioserosa cells of a dominant Inx2 mutant.
The image shows amnioserosa cells (As) and leading edge (LE) epidermal cells at a stage during early dorsal closure in an embryo of genotype Inx2TA181 / Y. Inx2 protein (green) is detected using anti-Inx2 rabbit polyclonal primary antibody and an FITC-conjugated anti-rabbit secondary. The mutant Inx2 protein fails to form discrete plaques at the cellular plasma membrane and distributes itself almost evenly throughout the membrane instead (arrowhead). There are a few intensely-staining, small spots that are Inx2-immunopositive. These may be transport vesicles (the mutant Inx2 protein is still transported to the cell membrane) or they may reflect a small amount of maternally contributed wild type Inx2.