Inx2 protein in the pupal brain.
A single optical section through the brain of a wild type golden pupa. Inx2 protein was detected using anti-Inx2 rabbit polyclonal primary antibody and an alexa488-conjugated anti-rabbit secondary antibody (green). Arrowheads in each hemisphere point to putative processes of the “transient interhemispheric fibrous ring” (TIFR), glial cells that exist only during the larval and pupal stages. Mutation of proteins expressed within the TIFR, such as linotte, are associated with defective adult mushroom body morphology ( Hitier et al. 2000 and 2001). The image is over-exposed in order to detect weaker staining within the brain. Because of this anti-Inx2 immunofluorescence at the brain periphery appears overly-bright and is not distinguishable to specific cells.