Inx2 protein distribution in a pupal leg.
An extending leg from a wild type golden pupa stained to detect Inx2 (red) and F-actin (yellow). Inx2 protein was detected using anti-Inx2 rabbit polyclonal primary antibody and a Cy5-conjugated anti-rabbit secondary (red). F- actin is detected using rhodamine-phalloidin conjugate (yellow). A, Inx2 staining alone. White arrowheads point to Inx2 in the peripodial membrane surrounding the developing leg. Blue arrowheads indicate Inx2 protein at one pole of epithelial cells. Inx2 has a polarized distribution within these cells just as it does in other cell types, such as salivary gland cells. B, merge of all staining.