Inx2 protein distribution in the pupal gut.
Gut from a wild type golden pupa stained to detect Inx2 (red), nuclei (blue) and F-actin (yellow). Inx2 protein was detected using anti-Inx2 rabbit polyclonal primary antibody and a Cy5-conjugated anti-rabbit secondary (red). Nuclei are stained with DAPI (blue) and F- actin is detected using rhodamine-phalloidin conjugate (yellow). A, Inx2 staining alone. B, merge of all staining.