Inx3 protein distribution in an inx2 null mutant embryo.
Images from the same inx2 null mutant embryo. A, focusing on the epidermis (Ep). Inx3 protein is expressed strongly in cells of the epidermis despite the absence of Inx2. Inx3 can no longer be detected at the borders of amnioserosa cells in images of this magnification because normal, large plaques, do not form. In this image there is possibly some evidence of holes developing in the epidermis (arrows) where antibody staining appears weaker than in surrounding tissue. Holes have been reported previously in inx2 mutants ( Bauer et al., 2004), however, the cuticles in all inx2 mutants analysed in this study were intact. Perhaps modifiers in the genetic background of different inx2 alleles have a significant effect on this phenotpye. It's also possible that the effect is artificial in this case, arising because the focal plane is beyond the epithelial cells in some areas of the image. B, focusing on the interior of the embryo. Inx3 protein is abundant in the salivary glands (Sal), Gut and Epidermis (Ep) – the same places it is found in wild type embryos. The embryo is 8-10 hours after egg laying at 25C. Genotype is inx2F43 /Y , which produces no Inx2 protein. Antibody staining using anti-Inx3 rabbit polyclonal primary antibody and an FITC-conjugated anti-rabbit secondary (green).