Innexin Gap Junctions

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whole embryo Inx2-antibody-staining - inx2 null mutant.

whole embryo Inx2-antibody-staining - inx2 null mutant.

The images show a fluorescent (left) and a bright field (right) image of the same inx2 null mutant, 8-10 hour old embryo. Genotype inx2F43/Y. The embryo was antibody stained to detect Inx2 protein using anti-Inx2 rabbit polyclonal primary antibody and an FITC-conjugated anti-rabbit secondary (green). All Inx2 protein is absent, confirming the molecular basis of the mutant as a null generated by transposon-mediated deletion. The inx2F43 deletion removes 1368bp of sequence from 32bp upstream of the transcription start site to 1336bp downstream of the transcription start site; it therefore removes the transcription start site and much of the protein coding sequence. Only background fluorescence and some gut auto-fluorescence can be detected suggesting that any maternally contributed Inx2 protein has expired in embryos of this age. The bright field image looks normal for an embryo of this age despite reports from other labs that inx2 zygotic null mutants exhibit large holes in the epidermis ( Bauer et al., 2004 Gap junction channel protein Innexin 2 is essential for epithelial morphogenesis in the Drosophila embryo).

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