Inx2 protein distribution in dominant mutant Inx2TA181 gut cells.
The image shows cells of the posterior embryonic gut of an 8-10 hour old embryo of genotype Inx2TA181 / Y. The mutant Inx2 protein (green) is detected using anti-Inx2 rabbit polyclonal primary antibody and an FITC-conjugated anti-rabbit secondary. The Inx2-staining intensity observed in cells of this genotype is always low, presumably because the protein is distributed around the cell membrane and does not aggregate into the brightly fluorescent plaques observed in wild type animals. That said, the staining is more intense than background staining (as observed when inx2null embryos are stained using the same technique) and this indicates that the Inx2TA181 mutant protein is produced in the appropriate cells. Phenotypes associated with the dominant mutation must therefore be due to aberrant activity of the synthesised protein.