Innexin Gap Junctions

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Disorganised mushroom body neuronal architecture in an ogre mutant.


Disorganised mushroom body neuronal architecture in an ogre mutant.

Various defective mushroom body (MB) architecture phenotypes are observed in ogre mutant genotype flies. Panels A and B show the MB lobe region of normal flies (control and wild type). All other figures are of ogre mutant brains. Images B, K and L show MBs stained using a primary antibody specific for FasII (purple). All other images show MB morphology revealed by expressing UAS-mCD8:GFP in a subset of MB neurons under the transcriptional direction of the 201YGAL (Yang et al, 1995) driver line. A, MB lobe morphology in a control genotype: Df(1)Sxlbt / +
-MB structure looks normal. The α and β/γ lobe positions are labelled and a dashed line indicates axons of the median bundle at the brain midline. All subsequent images are in the same orientation. B, FasII staining of a wild type brain reveals a greater level of staining than 201yGAL4 in all three lobes, α, γ and the β-lobe as well as some non-MB neurons. C, few ogre mutant MB axons express mCD8:GFP at wild type levels in most of the brains examined. Here, some axons expressing GFP at normal levels cross the midline (arrow). D, Very weakly staining axons. Abnormally shaped β/γ lobes fuse across midline (arrow). E, Multiple neuronal fascicles traverse the midline (arrows). α lobe is thin and miss-shapen towards tip (*). F, β/γ lobe axons extend in abnormal directions (arrows) and cross the midline. α lobe is missing. k-Kenyon cell bodies. p-pedunculus. G, A single strongly staining axon bifurcates at the base of the pedunculus (arrow) but both processes enter the α lobe. Axons then travel in abnormal directions (arrowheads). Abnormally large spots of mCD8:GFP appear along axons (*). H, MB axon bifurcates normally (arrow) extending a process into the α and β/γ lobes. Anomalous U-turn occurs in the β/γ lobe axon (arrowheads). I, α lobe has abnormal shape(*). mCD8:GFP has punctate pattern in calyx (c) and at tips of β/γ lobes (arrow) and at the base of the pedunculus (p). J, β/γ lobes fuse across midline in mass of mCD8:GFP spots. Tip of α lobe has irregular outgrowth (*). Inset shows another α lobe outgrowth extending in an arc from the tip of the α lobe. K, FasII staining is often weak in ogre mutant flies. α lobe tip is miss-shapen (*) and β/γ lobes bunch up (arrow, phenotype occurs in 9/66=14% of brains). FasII immunoreactivity in the mutant often appears spotty. Inset, arc shaped axonal outgrowth at tip of α lobe. L, FasII staining reveals two strongly staining median tracts (arrowheads). Possibly α lobe axons parallel to the β lobe. The α lobe is spotty and almost devoid of normal axonal projections. Size bars are 20μm.



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